Authors: Yogeswaran Umasankar, Mubarak Mujawar, Shekhar Bhansali
Publication Date: 2018/10/28
Conference: 2018 IEEE SENSORS
Pages: 1-4
Publisher: IEEE
Description: This work presents an approach towards integrating enzymatic biosensors to monitor wound healing. Levels of uric acid (UA) a metabolic byproduct of purine degradation in dying cells, is investigated as an indicator of wound healing. The biosensor was fabricated by immobilizing uricase (UOx) natural catalyst for UA as the enzyme and, multi-walled carbon nanotubes (MWNT) and gold nanoparticles (Au) as electron exchange matrix. UA is oxidized by UOx with the Au nanoparticle and MWNT matrix providing thermodynamically feasible electron transfer pathways leading to the detection of byproduct H 2 O 2 , through its reduction on the matrix. This matrix was treated with cold plasma treatment which enabled sintering of Au nanoparticles on MWNTs, without damaging the substrate. This sintering induced densification led to higher conductivity of the matrix. The electrochemical results show that the treatment …